![Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177605/369461/STJ11104961_1__90609.1713134964.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of HepG2 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177605/369462/STJ11104961_2__74130.1713134964.jpg?c=1 "Immunofluorescence analysis of HepG2 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of HeLa cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177605/369463/STJ11104961_3__34185.1713134965.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of paraffin-embedded mouse testis using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177605/369464/STJ11104961_4__41743.1713134966.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded mouse testis using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of paraffin-embedded human liver using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177605/369465/STJ11104961_5__75755.1713134966.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded human liver using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177605/369466/STJ11104961_6__38319.1713134967.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177605/369461/STJ11104961_1__90609.1713134964.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.")
Anti-COX4I1 antibody (23-98) (STJ11104961)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Cytochrome C Oxidase Subunit 4 Isoform 1-Mitochondrial (23-98) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COX4I1 |
| Gene ID | 1327 |
| Uniprot ID | COX41_HUMAN |
| Immunogen Region | 23-98 |
| Immunogen Sequence | AHESVVKSEDFSLPAYMDRR DHPLPEVAHVKHLSASQKAL KEKEKASWSSLSMDEKVELY RIKFKESFAEMNRGSN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 23-98 of human COX IV. (NP_001852.1). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. |
| Protein Name | Cytochrome C Oxidase Subunit 4 Isoform 1 - MitochondrialCytochrome C Oxidase Polypeptide IvCytochrome C Oxidase Subunit Iv Isoform 1Cox Iv-1 |
| Database Links | Reactome: R-HSA-5628897Reactome: R-HSA-611105Reactome: R-HSA-9707564Reactome: R-HSA-9837999Reactome: R-HSA-9864848 |
| Cellular Localisation | Mitochondrion Inner MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome C Oxidase Subunit 4 Isoform 1 - Mitochondrial antibodyAnti-Cytochrome C Oxidase Polypeptide Iv antibodyAnti-Cytochrome C Oxidase Subunit Iv Isoform 1 antibodyAnti-Cox Iv-1 antibodyAnti-COX4I1 antibodyAnti-COX4 antibody |
Information sourced from Uniprot.org