• Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KD Validated] COX IV Rabbit polyclonal antibody (STJ11104961) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from wild type (WT) and COX IVknockdown (KD) 293T (KD) cells, using COX IV antibody (STJ11104961) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates, using COX IV antibody (STJ11104961) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-COX4I1 antibody (23-98) (STJ11104961)

SKU:
STJ11104961

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cytochrome C Oxidase Subunit 4 Isoform 1-Mitochondrial (23-98) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COX4I1
Gene ID: 1327
Uniprot ID: COX41_HUMAN
Immunogen Region: 23-98
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-98 of human COX IV. (NP_001852.1).
Immunogen Sequence: AHESVVKSEDFSLPAYMDRR DHPLPEVAHVKHLSASQKAL KEKEKASWSSLSMDEKVELY RIKFKESFAEMNRGSN
Tissue Specificity Ubiquitous.
Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Protein Name Cytochrome C Oxidase Subunit 4 Isoform 1 - Mitochondrial
Cytochrome C Oxidase Polypeptide Iv
Cytochrome C Oxidase Subunit Iv Isoform 1
Cox Iv-1
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 4 Isoform 1 - Mitochondrial antibody
Anti-Cytochrome C Oxidase Polypeptide Iv antibody
Anti-Cytochrome C Oxidase Subunit Iv Isoform 1 antibody
Anti-Cox Iv-1 antibody
Anti-COX4I1 antibody
Anti-COX4 antibody

Information sourced from Uniprot.org

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