Anti-COX15 antibody (150-250) (STJ116869)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal COX15 (150-250) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COX15 |
| Gene ID | 1355 |
| Uniprot ID | COX15_HUMAN |
| Immunogen Region | 150-250 |
| Immunogen Sequence | YSHRMWGRLVGLVYILPAAY FWRKGWLSRGMKGRVLALCG LVCFQGLLGWYMVKSGLEEK SDSHDIPRVSQYRLAAHLGS ALVLYCASLWTSLSLLLPPH K |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human COX15 (NP_510870.1). |
Additional Info
| Tissue Specificity | Predominantly found in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle, heart, and brain. |
| Function | Catalyzes the second reaction in the biosynthesis of heme A, a prosthetic group of mitochondrial cytochrome c oxidase (CcO). Heme A is synthesized from heme B by two sequential enzymatic reactions catalyzed by heme O synthase (HOS) and heme A synthase (HAS). HAS catalyzes the conversion of heme O to heme A by two successive hydroxylations of the methyl group at C8, in a reaction that involves matrix ferredoxin and ferredoxin reductase. The first hydroxylation forms heme I, the second hydroxylation results in an unstable dihydroxymethyl group, which spontaneously dehydrates, resulting in the formyl group of heme A. |
| Protein Name | Heme A Synthase Cox15HasCytochrome C Oxidase Assembly Protein Cox15 Homolog |
| Database Links | Reactome: R-HSA-189451Reactome: R-HSA-9864848 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Heme A Synthase Cox15 antibodyAnti-Has antibodyAnti-Cytochrome C Oxidase Assembly Protein Cox15 Homolog antibodyAnti-COX15 antibody |
Information sourced from Uniprot.org