• Immunohistochemistry analysis of paraffin-embedded mouse lung using COTL1 Rabbit polyclonal antibody (STJ23203) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using COTL1 Rabbit polyclonal antibody (STJ23203) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using COTL1 Rabbit polyclonal antibody (STJ23203) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using COTL1 antibody (STJ23203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COTL1 antibody (1-142) (STJ23203)

SKU:
STJ23203

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COTL1 (1-142) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COTL1
Gene ID: 23406
Uniprot ID: COTL1_HUMAN
Immunogen Region: 1-142
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human COTL1 (NP_066972.1).
Immunogen Sequence: MATKIDKEACRAAYNLVRDD GSAVIWVTFKYDGSTIVPGE QGAEYQHFIQQCTDDVRLFA FVRFTTGDAMSKRSKFALIT WIGENVSGLQRAKTGTDKTL VKEVVQNFAKEFVISDRKEL EEDFIKSELKKAGGANYDAQ TE
Tissue Specificity Widely expressed with highest levels in placenta, lung, kidney and peripheral blood leukocytes and lower levels in brain, liver and pancreas.
Function Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.
Protein Name Coactosin-Like Protein
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Alternative Antibody Names Anti-Coactosin-Like Protein antibody
Anti-COTL1 antibody
Anti-CLP antibody

Information sourced from Uniprot.org

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