• Immunofluorescence analysis of U2OS cells using COPB2 Rabbit polyclonal antibody (STJ29116) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using COPB2 Rabbit polyclonal antibody (STJ29116) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using COPB2 Rabbit polyclonal antibody (STJ29116) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of COPB2 in paraffin-embedded mouse spinal cord using COPB2 Rabbit polyclonal antibody (STJ29116) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of COPB2 in paraffin-embedded human colon carcinoma using COPB2 Rabbit polyclonal antibody (STJ29116) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of COPB2 in paraffin-embedded human breast cancer using COPB2 Rabbit polyclonal antibody (STJ29116) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using COPB2 Rabbit polyclonal antibody (STJ29116) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-COPB2 antibody (657-906) (STJ29116)

SKU:
STJ29116

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COPB2 (657-906) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COPB2
Gene ID: 9276
Uniprot ID: COPB2_HUMAN
Immunogen Region: 657-906
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1).
Immunogen Sequence: AYQLAVEAESEQKWKQLAEL AISKCQFGLAQECLHHAQDY GGLLLLATASGNANMVNKLA EGAERDGKNNVAFMSYFLQG KVDACLELLIRTGRLPEAAF LARTYLPSQVSRVVKLWREN LSKVNQKAAESLADPTEYEN LFPGLKEAFVVEEWVKETHA DLWPAKQYPLVTPNEERNVM EEGKDFQPSRSTAQQELDGK PASPTPVIVASHTANKEEKS LLELEVDLDNLELEDIDTT
Function The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins.the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.
Protein Name Coatomer Subunit Beta'
Beta'-Coat Protein
Beta'-Cop
P102
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Cytoplasm
Cytosol
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Copi-Coated Vesicle Membrane
The Coatomer Is Cytoplasmic Or Polymerized On The Cytoplasmic Side Of The Golgi
As Well As On The Vesicles/Buds Originating From It
Shows Only A Slight Preference For The Cis-Golgi Apparatus
Compared With The Trans-Golgi
Alternative Antibody Names Anti-Coatomer Subunit Beta' antibody
Anti-Beta'-Coat Protein antibody
Anti-Beta'-Cop antibody
Anti-P102 antibody
Anti-COPB2 antibody

Information sourced from Uniprot.org

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