• Immunohistochemistry analysis of COPA in paraffin-embedded mouse brain tissue using COPA Rabbit monoclonal antibody (STJ11103071) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COPA in paraffin-embedded human colon carcinoma tissue using COPA Rabbit monoclonal antibody (STJ11103071) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COPA in paraffin-embedded human colon tissue using COPA Rabbit monoclonal antibody (STJ11103071) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COPA in paraffin-embedded human thyroid cancer tissue using COPA Rabbit monoclonal antibody (STJ11103071) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COPA in paraffin-embedded mouse testis tissue using COPA Rabbit monoclonal antibody (STJ11103071) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COPA in paraffin-embedded rat colon tissue using COPA Rabbit monoclonal antibody (STJ11103071) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Rat brain, using COPA Rabbit monoclonal antibody (STJ11103071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using COPA Rabbit monoclonal antibody (STJ11103071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-COPA antibody (1-100) [S1MR] (STJ11103071)

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STJ11103071

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-COPA (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COPA
Gene ID: 1314
Uniprot ID: COPA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human COPA (P53621).
Immunogen Sequence: MLTKFETKSARVKGLSFHPK RPWILTSLHNGVIQLWDYRM CTLIDKFDEHDGPVRGIDFH KQQPLFVSGGDDYKIKVWNY KLRRCLFTLLGHLDYIRTTF
Tissue Specificity Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.
Function The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins.the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.
Protein Name Coatomer Subunit Alpha
Alpha-Coat Protein
Alpha-Cop
Hep-Cop
Hepcop Cleaved Into - Xenin
Xenopsin-Related Peptide - Proxenin
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Copi-Coated Vesicle Membrane
The Coatomer Is Cytoplasmic Or Polymerized On The Cytoplasmic Side Of The Golgi
As Well As On The Vesicles/Buds Originating From It
Xenin: Secreted
Alternative Antibody Names Anti-Coatomer Subunit Alpha antibody
Anti-Alpha-Coat Protein antibody
Anti-Alpha-Cop antibody
Anti-Hep-Cop antibody
Anti-Hepcop Cleaved Into - Xenin antibody
Anti-Xenopsin-Related Peptide - Proxenin antibody
Anti-COPA antibody

Information sourced from Uniprot.org

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