• Immunohistochemistry analysis of Collagen II. Sample: mouse bone, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2600, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of Collagen II. Sample: mouse bone, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2600, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of Collagen II. Sample: Rat bone, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:2600, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-Collagen II antibody [SM5170] (STJA0005170)

SKU:
STJA0005170

£170.50 - £233.50
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Host: Mouse
Applications: IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Collagen II is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM5170
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: IHC/IF (M, R) 1:700-1:2500/1:700-1:1400
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Col2a1
Gene ID: 12824
Uniprot ID: CO2A1_MOUSE
Immunogen: Recombinant protein corresponding to Mouse collagen II
Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Protein Name Collagen Alpha-1(Ii Chain
Alpha-1 Type Ii Collagen Cleaved Into - Collagen Alpha-1(Ii Chain - Chondrocalcin
Database Links Reactome: R-MMU-1442490
Reactome: -MMU-1474244
Reactome: -MMU-1650814
Reactome: -MMU-186797
Reactome: -MMU-198933
Reactome: -MMU-2022090
Reactome: -MMU-216083
Reactome: -MMU-3000171
Reactome: -MMU-3000178
Reactome: -MMU-419037
Reactome: -MMU-8874081
Reactome: -MMU-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Ii Chain antibody
Anti-Alpha-1 Type Ii Collagen Cleaved Into - Collagen Alpha-1(Ii Chain - Chondrocalcin antibody
Anti-Col2a1 antibody

Information sourced from Uniprot.org

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