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Anti-COL1A2 antibody (1-50 aa) (STJ92383)

Immunofluorescence analysis of NIH/3T3 cells, using Collagen I Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen I Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, COL1A2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using COL1A2 Polyclonal Antibody diluted at 1：500
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, COL1A2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-lung tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-stomach tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-stomach tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-COL1A2 antibody (1-50 aa) (STJ92383)

SKU:

STJ92383

$62.10 - $305.10

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Collagen alpha-2I chain (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	COL1A2
Gene ID:	1278
Uniprot ID:	CO1A2_HUMAN
Immunogen Region:	1-50 aa
Specificity:	COL1A2 Polyclonal Antibody detects endogenous levels of COL1A2 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Collagen I at the amino acid range 1-50

Post Translational Modifications	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function	Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name	Collagen Alpha-2(I Chain Alpha-2 Type I Collagen
Database Links	Reactome: R-HSA-114604 Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-198933 Reactome: R-HSA-2022090 Reactome: R-HSA-202733 Reactome: R-HSA-216083 Reactome: R-HSA-2173796 Reactome: R-HSA-2214320 Reactome: R-HSA-2243919 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-3000480 Reactome: R-HSA-430116 Reactome: R-HSA-6785807 Reactome: R-HSA-75892 Reactome: R-HSA-76009 Reactome: R-HSA-8874081 Reactome: R-HSA-8948216
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Collagen Alpha-2(I Chain antibody Anti-Alpha-2 Type I Collagen antibody Anti-COL1A2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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