• Western blot analysis of various lysates using Coilin Rabbit polyclonal antibody (STJ28511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COIL antibody (297-576) (STJ28511)

SKU:
STJ28511

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Coilin (297-576) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COIL
Gene ID: 8161
Uniprot ID: COIL_HUMAN
Immunogen Region: 297-576
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 297-576 of human Coilin (NP_004636.1).
Immunogen Sequence: KLGFSLTPSKGKTSGTTSSS SDSSAESDDQCLMSSSTPEC AAGFLKTVGLFAGRGRPGPG LSSQTAGAAGWRRSGSNGGG QAPGASPSVSLPASLGRGWG REENLFSWKGAKGRGMRGRG RGRGHPVSCVVNRSTDNQRQ QQLNDVVKNSSTIIQNPVET PKKDYSLLPLLAAAPQVGEK IAFKLLELTSSYSPDVSDYK EGRILSHNPETQQVDIEILS SLPALREPGKFDLVYHNEN
Tissue Specificity Found in all the cell types examined.
Post Translational Modifications Symmetrical dimethylation of arginine residues within the RG repeat region enhances affinity for SMN, and thus localization of SMN complexes to CBs. Phosphorylation during mitosis is associated with disassembly of CBs.
Function Component of nuclear coiled bodies, also known as Cajal bodies or CBs, which are involved in the modification and assembly of nucleoplasmic snRNPs.
Protein Name Coilin
P80-Coilin
Cellular Localisation Nucleus
Cajal Body
Alternative Antibody Names Anti-Coilin antibody
Anti-P80-Coilin antibody
Anti-COIL antibody
Anti-CLN80 antibody

Information sourced from Uniprot.org

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