• Immunohistochemistry analysis of paraffin-embedded mouse kidney using COG2 antibody (STJ28007) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using COG2 antibody (STJ28007) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using COG2 antibody (STJ28007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 80s.

Anti-COG2 antibody (1-280) (STJ28007)

SKU:
STJ28007

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COG2 (1-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COG2
Gene ID: 22796
Uniprot ID: COG2_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human COG2 (NP_031383.1).
Immunogen Sequence: MEKSRMNLPKGPDTLCFDKD EFMKEDFDVDHFVSDCRKRV QLEELRDDLELYYKLLKTAM VELINKDYADFVNLSTNLVG MDKALNQLSVPLGQLREEVL SLRSSVSEGIRAVDERMSKQ EDIRKKKMCVLRLIQVIRSV EKIEKILNSQSSKETSALEA SSPLLTGQILERIATEFNQL QFHAVQSKGMPLLDKVRPRI AGITAMLQQSLEGLLLEGLQ TSDVDIIRHCLRTYATIDK
Function Required for normal Golgi morphology and function.
Protein Name Conserved Oligomeric Golgi Complex Subunit 2
Cog Complex Subunit 2
Component Of Oligomeric Golgi Complex 2
Low Density Lipoprotein Receptor Defect C-Complementing Protein
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811438
Reactome: R-HSA-6811440
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Conserved Oligomeric Golgi Complex Subunit 2 antibody
Anti-Cog Complex Subunit 2 antibody
Anti-Component Of Oligomeric Golgi Complex 2 antibody
Anti-Low Density Lipoprotein Receptor Defect C-Complementing Protein antibody
Anti-COG2 antibody
Anti-LDLC antibody

Information sourced from Uniprot.org

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