| Host: | Rabbit |
| Applications: | IHC-P/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | IHC-P:1:50-1:100IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | COCH |
| Gene ID: | 1690 |
| Uniprot ID: | COCH_HUMAN |
| Immunogen Region: | 20-260 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-260 of human COCH (NP_001128530.1). |
| Immunogen Sequence: | GPAGSEGAAPIAITCFTRGL DIRKEKADVLCPGGCPLEEF SVYGNIVYASVSSICGAAVH RGVISNSGGPVRVYSLPGRE NYSSVDANGIQSQMLSRWSA SFTVTKGKSSTQEATGQAVS TAHPPTGKRLKKTPEKKTGN KDCKADIAFLIDGSFNIGQR RFNLQKNFVGKVALMLGIGT EGPHVGLVQASEHPKIEFYL KNFTSAKDVLFAIKEVGFRG GNSNTGKALKHTAQKFFTV |
| Tissue Specificity | Expressed in inner ear structures.the cochlea and the vestibule. |
| Post Translational Modifications | N-glycosylated. A 50 kDa form is created by proteolytic cleavage. |
| Function | Plays a role in the control of cell shape and motility in the trabecular meshwork. |
| Protein Name | CochlinCoch-5b2 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Cochlin antibodyAnti-Coch-5b2 antibodyAnti-COCH antibodyAnti-COCH5B2 antibodyAnti-UNQ257 antibodyAnti-PRO294 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89724/358205/STJ11100788_1__51624.1713122526.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and APEH knockout (KO) 293T cells, using [KO Validated] APEH Rabbit polyclonal antibody (STJ11100788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
