• Western blot analysis of extracts of various cell lines, using CNPY3 antibody (STJ29256) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 15s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using CNPY3 antibody (STJ29256) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300ug extracts of THP-1 cells using 3ug CNPY3 rabbit polyclonal antibody (STJ29256 1:40). Western blot was performed from the immunoprecipitate using CNPY3 rabbit polyclonal antibody (STJ29256) at a dilition of 1:500.

Anti-CNPY3 antibody (31-278) (STJ29256)

SKU:
STJ29256

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNPY3 (31-278) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNPY3
Gene ID: 10695
Uniprot ID: CNPY3_HUMAN
Immunogen Region: 31-278
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 31-278 of human CNPY3 (NP_006577.2).
Immunogen Sequence: GPSQAGAEENDWVRLPSKCE VCKYVAVELKSAFEETGKTK EVIGTGYGILDQKASGVKYT KSDLRLIEVTETICKRLLDY SLHKERTGSNRFAKGMSETF ETLHNLVHKGVKVVMDIPYE LWNETSAEVADLKKQCDVLV EEFEEVIEDWYRNHQEEDLT EFLCANHVLKGKDTSCLAEQ WSGKKGDTAALGGKKSKKKS SRAKAAGGRSSSSKQRKELG GLEGDPSPEEDEGIQKASP
Function Toll-like receptor (TLR)-specific co-chaperone for HSP90B1. Required for proper TLR folding, except that of TLR3, and hence controls TLR exit from the endoplasmic reticulum. Consequently, required for both innate and adaptive immune responses.
Protein Name Protein Canopy Homolog 3
Ctg Repeat Protein 4a
Expanded Repeat-Domain Protein Cag/Ctg 5
Protein Associated With Tlr4
Trinucleotide Repeat-Containing Gene 5 Protein
Database Links Reactome: R-HSA-1679131
Cellular Localisation Endoplasmic Reticulum
Alternative Antibody Names Anti-Protein Canopy Homolog 3 antibody
Anti-Ctg Repeat Protein 4a antibody
Anti-Expanded Repeat-Domain Protein Cag/Ctg 5 antibody
Anti-Protein Associated With Tlr4 antibody
Anti-Trinucleotide Repeat-Containing Gene 5 Protein antibody
Anti-CNPY3 antibody
Anti-CTG4A antibody
Anti-ERDA5 antibody
Anti-PRAT4A antibody
Anti-TNRC5 antibody
Anti-HSPC084 antibody
Anti-UNQ1934 antibody
Anti-PRO4409 antibody

Information sourced from Uniprot.org

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