• Immunofluorescence analysis of U2OS cells using CNN3 Rabbit polyclonal antibody (STJ11103277) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using CNN3 Rabbit polyclonal antibody (STJ11103277) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CNN3 antibody (STJ11103277) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CNN3 antibody (220-329) (STJ11103277)

SKU:
STJ11103277

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CNN3 (220-329) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CNN3
Gene ID: 1266
Uniprot ID: CNN3_HUMAN
Immunogen Region: 220-329
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-329 of human CNN3 (NP_001830.1).
Immunogen Sequence: LAPGTRRDIYDQKLTLQPVD NSTISLQMGTNKVASQKGMS VYGLGRQVYDPKYCAAPTEP VIHNGSQGTGTNGSEISDSD YQAEYPDEYHGEYQDDYPRD YQYSDQGIDY
Tissue Specificity Expressed in both non-smooth muscle tissues as well as smooth muscle tissues.
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Protein Name Calponin-3
Calponin - Acidic Isoform
Alternative Antibody Names Anti-Calponin-3 antibody
Anti-Calponin - Acidic Isoform antibody
Anti-CNN3 antibody

Information sourced from Uniprot.org

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