• Western blot analysis of extracts of various cell lines, using CLTB antibody (STJ110702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HeLa cells using CLTB Polyclonal Antibody (STJ110702) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using CLTB Polyclonal Antibody (STJ110702) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CLTB antibody (STJ110702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CLTB antibody (1-211) (STJ110702)

SKU:
STJ110702

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLTB (1-211) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLTB
Gene ID: 1212
Uniprot ID: CLCB_HUMAN
Immunogen Region: 1-211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLTB (NP_001825.1).
Immunogen Sequence: MADDFGFFSSSESGAPEAAE EDPAAAFLAQQESEIAGIEN DEGFGAPAGSHAAPAQPGPT SGAGSEDMGTTVNGDVFQEA NGPADGYAAIAQADRLTQEP ESIRKWREEQRKRLQELDAA SKVTEQEWREKAKKDLEEWN QRQSEQVEKNKINNRASEEA FVKESKEETPGTEWEKVAQL CDFNPKSSKQCKDVSRLRSV LMSLKQTPLSR
Function Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Protein Name Clathrin Light Chain B
Lcb
Database Links Reactome: R-HSA-190873
Reactome: R-HSA-196025
Reactome: R-HSA-3928665
Reactome: R-HSA-432720
Reactome: R-HSA-5099900
Reactome: R-HSA-5140745
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Coated Pit
Cytoplasmic Face Of Coated Pits And Vesicles
Alternative Antibody Names Anti-Clathrin Light Chain B antibody
Anti-Lcb antibody
Anti-CLTB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance