| Short Description : | Goat polyclonal antibody anti-CLO29 (30-43) is suitable for use in ELISA and Western Blot research applications. |
| Applications: | Pep-ELISA/WB |
| Host: | Goat |
| Reactivity: | Human/Pig/Sheep/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 38kDa band observed in lysates of cell line HeLa (calculated MW of 37.5kDa according to NP_001009894.2). Recommended concentration: 1-3µg/ml. |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | RLIG1 |
| Gene ID: | 91298 |
| Uniprot ID: | RLIG1_HUMAN |
| Accession Number: | NP_001009894.2 |
| Immunogen Region: | 30-43 |
| Immunogen Sequence: | QPFKVLATETVSHK |
| Specificity: | This antibody is expected to recognize isoform 1 (Q8N999-1) and isoform 3 (Q8N999-3) as descibed in UniprotKB. |
| Function | Functions as an RNA ligase, in vitro. The ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate. In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP. Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids. May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS). |
| Protein Name | Rna Ligase 1Rna LigaseRnl |
| Alternative Antibody Names | Anti-Rna Ligase 1 antibodyAnti-Rna Ligase antibodyAnti-Rnl antibodyAnti-RLIG1 antibodyAnti-C12orf29 antibody |
Information sourced from Uniprot.org
