• Western blot analysis of extracts of various cell lines, using CLNS1A antibody (STJ111810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using CLNS1A Rabbit polyclonal antibody (STJ111810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CLNS1A antibody (1-237) (STJ111810)

SKU:
STJ111810

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLNS1A (1-237) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLNS1A
Gene ID: 1207
Uniprot ID: ICLN_HUMAN
Immunogen Region: 1-237
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-237 of human CLNS1A (NP_001284.1).
Immunogen Sequence: MSFLKSFPPPGPAEGLLRQQ PDTEAVLNGKGLGTGTLYIA ESRLSWLDGSGLGFSLEYPT ISLHALSRDRSDCLGEHLYV MVNAKFEEESKEPVADEEEE DSDDDVEPITEFRFVPSDKS ALEAMFTAMCECQALHPDPE DEDSDDYDGEEYDVEAHEQG QGDIPTFYTYEEGLSHLTAE GQATLERLEGMLSQSVSSQY NMAGVRTEDSIRDYEDGMEV DTTPTVAGQFEDADVDH
Function Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.
Protein Name Methylosome Subunit Picln
Chloride Channel - Nucleotide Sensitive 1a
Chloride Conductance Regulatory Protein Icln
I(Cln
Chloride Ion Current Inducer Protein
Clci
Reticulocyte Picln
Database Links Reactome: R-HSA-191859
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Cytoskeleton
A Small Fraction Is Also Associated With The Cytoskeleton
Alternative Antibody Names Anti-Methylosome Subunit Picln antibody
Anti-Chloride Channel - Nucleotide Sensitive 1a antibody
Anti-Chloride Conductance Regulatory Protein Icln antibody
Anti-I(Cln antibody
Anti-Chloride Ion Current Inducer Protein antibody
Anti-Clci antibody
Anti-Reticulocyte Picln antibody
Anti-CLNS1A antibody
Anti-CLCI antibody
Anti-ICLN antibody

Information sourced from Uniprot.org

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