• Western blot analysis of extracts of various cell lines, using CLIP1 antibody (STJ114372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CLIP1 antibody (1228-1427) (STJ114372)

SKU:
STJ114372

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLIP1 (1228-1427) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLIP1
Gene ID: 6249
Uniprot ID: CLIP1_HUMAN
Immunogen Region: 1228-1427
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1228-1427 of human CLIP1 (NP_002947.1).
Immunogen Sequence: SALLTEKDAELEKLRNEVTV LRGENASAKSLHSVVQTLES DKVKLELKVKNLELQLKENK RQLSSSSGNTDTQADEDERA QESQIDFLNSVIVDLQRKNQ DLKMKVEMMSEAALNGNGDD LNNYDSDDQEKQSKKKPRLF CDICDCFDLHDTEDCPTQAQ MSEDPPHSTHHGSRGEERPY CEICEMFGHWATNCNDDETF
Tissue Specificity Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.
Post Translational Modifications Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons. Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules.
Function Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
Protein Name Cap-Gly Domain-Containing Linker Protein 1
Cytoplasmic Linker Protein 1
Cytoplasmic Linker Protein 170 Alpha-2
Clip-170
Reed-Sternberg Intermediate Filament-Associated Protein
Restin
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5626467
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Cytoskeleton
Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Ruffle
Localizes To Microtubule Plus Ends
Localizes Preferentially To The Ends Of Tyrosinated Microtubules
Accumulates In Plasma Membrane Regions With Ruffling And Protrusions
Associates With The Membranes Of Intermediate Macropinocytic Vesicles
Alternative Antibody Names Anti-Cap-Gly Domain-Containing Linker Protein 1 antibody
Anti-Cytoplasmic Linker Protein 1 antibody
Anti-Cytoplasmic Linker Protein 170 Alpha-2 antibody
Anti-Clip-170 antibody
Anti-Reed-Sternberg Intermediate Filament-Associated Protein antibody
Anti-Restin antibody
Anti-CLIP1 antibody
Anti-CYLN1 antibody
Anti-RSN antibody

Information sourced from Uniprot.org

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