Anti-CLIC1/NCC27 antibody (Internal) (STJ73390)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CLIC1/NCC27 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30kDa band observed in lysates of cell line U937 (calculated MW of 26.9kDa according to NP_001279.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.IHC |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CLIC1 |
| Gene ID | 1192 |
| Uniprot ID | CLIC1_HUMAN |
| Accession Number | NP_001279.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | TEVHTDTNKIEE |
Additional Info
| Post Translational Modifications | Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59. |
| Function | In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response. Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle. |
| Protein Name | Chloride Intracellular Channel Protein 1Chloride Channel AbpGlutaredoxin-Like Oxidoreductase Clic1Glutathione-Dependent Dehydroascorbate Reductase Clic1Nuclear Chloride Ion Channel 27Ncc27Regulatory Nuclear Chloride Ion Channel ProteinHrncc |
| Cellular Localisation | NucleusNucleus MembraneSingle-Pass Membrane ProteinCytoplasmCell MembraneEndoplasmic ReticulumMostly In The Nucleus Including In The Nuclear MembraneSmall Amount In The Cytoplasm And The Plasma MembraneExists Both As Soluble Cytoplasmic Protein And As Membrane Protein With Probably A Single Transmembrane DomainMight Not Be Present In The Nucleus Of Cardiac Cells |
| Alternative Antibody Names | Anti-Chloride Intracellular Channel Protein 1 antibodyAnti-Chloride Channel Abp antibodyAnti-Glutaredoxin-Like Oxidoreductase Clic1 antibodyAnti-Glutathione-Dependent Dehydroascorbate Reductase Clic1 antibodyAnti-Nuclear Chloride Ion Channel 27 antibodyAnti-Ncc27 antibodyAnti-Regulatory Nuclear Chloride Ion Channel Protein antibodyAnti-Hrncc antibodyAnti-CLIC1 antibodyAnti-G6 antibodyAnti-NCC27 antibody |
Information sourced from Uniprot.org