STJ71327 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-CLEC16A antibody (Internal) (STJ71327)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71327-100
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General Information

Short DescriptionGoat polyclonal antibody anti-CLEC16A (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:16000.
WB: Preliminary experiments gave an approx 70kDa band in Rat Testis lysates after 0.5ยตg/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolCLEC16A
Gene ID23274
Uniprot IDCL16A_HUMAN
Accession NumberNP_056041.1; NP_001230332.1
Immunogen RegionInternal
Immunogen SequenceSLENQDKGGERP

Additional Info

Function Regulator of mitophagy through the upstream regulation of the RNF41/NRDP1-PRKN pathway. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control. The RNF41/NRDP1-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. May protect RNF41/NRDP1 from proteasomal degradation, RNF41/NRDP1 which regulates proteasomal degradation of PRKN. Plays a key role in beta cells functions by regulating mitophagy/autophagy and mitochondrial health.
Protein Name Protein Clec16a
C-Type Lectin Domain Family 16 Member A
Cellular Localisation Endosome Membrane
Peripheral Membrane Protein
Lysosome Membrane
Associates With The Endolysosome Membrane
Alternative Antibody Names Anti-Protein Clec16a antibody
Anti-C-Type Lectin Domain Family 16 Member A antibody
Anti-CLEC16A antibody
Anti-KIAA0350 antibody

Information sourced from Uniprot.org

Citations

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