Anti-Cleaved-NOTCH3-Val1662 antibody (1661-1662) (STJA0005905)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human NOTCH3 (Cleaved-Val1662)
General Information
| Short Description | Rabbit polyclonal anti-Cleaved-Neurogenic locus notch homolog protein 3-Val1662 (1661-1662) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NOTCH3 |
| Gene ID | 4854 |
| Uniprot ID | NOTC3_HUMAN |
| Immunogen | Synthesized peptide derived from human NOTCH3 (Cleaved-Val1662) |
| Immunogen Region | 1661-1662 |
| Specificity | This antibody detects endogenous levels of Human, Mouse, Rat NOTCH3 (Cleaved-Val1662, protein was cleaved amino acid sequence between 1661-1662 ) |
Additional Info
| Tissue Specificity | Ubiquitously expressed in fetal and adult tissues. |
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. Hydroxylated by HIF1AN. |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 3Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain |
| Database Links | Reactome: R-HSA-1912399Reactome: R-HSA-1912408Reactome: R-HSA-1912420Reactome: R-HSA-350054Reactome: R-HSA-5083630Reactome: R-HSA-9013507Reactome: R-HSA-9013508Reactome: R-HSA-9017802 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinNotch 3 Intracellular Domain: NucleusFollowing Proteolytical Processing Nicd Is Translocated To The Nucleus |
| Alternative Antibody Names | Anti-Neurogenic Locus Notch Homolog Protein 3 antibodyAnti-Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain antibodyAnti-NOTCH3 antibody |
Information sourced from Uniprot.org