Anti-CLCN2 antibody (C-Term) (STJ13100942)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from c-terminal region of human Chloride channel protein 2 (CLCN2) conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-CLCN2 (C-Term) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | CLCN2 |
| Gene ID | 1181 |
| Uniprot ID | CLCN2_HUMAN |
| Immunogen | A synthetic peptide from c-terminal region of human Chloride channel protein 2 (CLCN2) conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | C-Term |
| Specificity | Specific for CLCN2. |
Additional Info
| Post Translational Modifications | Phosphorylated. Activated by dephosphorylation. |
| Function | Voltage-gated and osmosensitive chloride channel. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Displays inward rectification currents activated upon membrane hyperpolarization and extracellular hypotonicity. Contributes to chloride conductance involved in neuron excitability. In hippocampal neurons, generates a significant part of resting membrane conductance and provides an additional chloride efflux pathway to prevent chloride accumulation in dendrites upon GABA receptor activation. In glia, associates with the auxiliary subunit HEPACAM/GlialCAM at astrocytic processes and myelinated fiber tracts where it may regulate transcellular chloride flux buffering extracellular chloride and potassium concentrations. Regulates aldosterone production in adrenal glands. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated calcium channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis. Contributes to chloride conductance in retinal pigment epithelium involved in phagocytosis of shed photoreceptor outer segments and photoreceptor renewal. Conducts chloride currents at the basolateral membrane of epithelial cells with a role in chloride reabsorption rather than secretion. Permeable to small monovalent anions with chloride > thiocyanate > bromide > nitrate > iodide ion selectivity. |
| Protein Name | Chloride Channel Protein 2Clc-2 |
| Database Links | Reactome: R-HSA-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneCell ProjectionDendritic Spine MembraneAxonSorting To The Basolateral Membrane Is Mediated By Ap-1 Clathrin AdapterLocalizes At Axon Initial Segments And Dendritic Shaft And SpikesColocalizes With Hepacam And Gfap At Astrocyte End-Foot In Contact With Brain Capillaries And Other Glial Cells |
| Alternative Antibody Names | Anti-Chloride Channel Protein 2 antibodyAnti-Clc-2 antibodyAnti-CLCN2 antibody |
Information sourced from Uniprot.org