Anti-CLCN1 antibody (C-Term) (STJ13100941)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the c-terminal region of mouse Chloride channel protein 1 (CLCN1) conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-CLCN1 (C-Term) for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Clcn1 |
| Gene ID | 12723 |
| Uniprot ID | CLCN1_MOUSE |
| Immunogen | A synthetic peptide from the c-terminal region of mouse Chloride channel protein 1 (CLCN1) conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | C-Term |
| Specificity | Specific for CLCN1. |
Additional Info
| Function | Voltage-gated chloride channel involved in skeletal muscle excitability. Generates most of the plasma membrane chloride conductance in skeletal muscle fibers, stabilizes the resting membrane potential and contributes to the repolarization phase during action potential firing. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Has a significant open probability at muscle resting potential and is further activated upon membrane depolarization. Permeable to small monovalent anions with ion selectivity for chloride > thiocyanate > bromide > nitrate > iodide. |
| Protein Name | Chloride Channel Protein 1Clc-1Chloride Channel Protein - Skeletal Muscle |
| Database Links | Reactome: R-MMU-2672351 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinSarcolemmaT-Tubule |
| Alternative Antibody Names | Anti-Chloride Channel Protein 1 antibodyAnti-Clc-1 antibodyAnti-Chloride Channel Protein - Skeletal Muscle antibodyAnti-Clcn1 antibodyAnti-Clc1 antibody |
Information sourced from Uniprot.org