• Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g CKMT1B antibody (STJ23148). Western blot was performed from the immunoprecipitate using CKMT1B antibody (STJ23148) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using CKMT1B Rabbit polyclonal antibody (STJ23148) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using CKMT1B Rabbit polyclonal antibody (STJ23148) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CKMT1B Rabbit polyclonal antibody (STJ23148) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CKMT1B antibody (1-85) (STJ23148)

SKU:
STJ23148

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CKMT1B (1-85) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CKMT1A.CKMT1B
Gene ID: 1159/548596
Uniprot ID: KCRU_HUMAN
Immunogen Region: 1-85
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human CKMT1B (NP_066270.1).
Immunogen Sequence: MAGPFSRLLSARPGLRLLAL AGAGSLAAGFLLRPEPVRAA SERRRLYPPSAEYPDLRKHN NCMASHLTPAVYARLCDKTT PTGWT
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Protein Name Creatine Kinase U-Type - Mitochondrial
Acidic-Type Mitochondrial Creatine Kinase
Mia-Ck
Ubiquitous Mitochondrial Creatine Kinase
U-Mtck
Database Links Reactome: R-HSA-71288
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Intermembrane Side
Alternative Antibody Names Anti-Creatine Kinase U-Type - Mitochondrial antibody
Anti-Acidic-Type Mitochondrial Creatine Kinase antibody
Anti-Mia-Ck antibody
Anti-Ubiquitous Mitochondrial Creatine Kinase antibody
Anti-U-Mtck antibody
Anti-CKMT1A antibody
Anti-CKMT.CKMT1B antibody
Anti-CKMT antibody

Information sourced from Uniprot.org

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