Anti-CKB/Brain Creatine Kinase antibody (Internal) (STJ70892)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CKB/Brain Creatine Kinase (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 45-48kDa band observed in human brain lysates (calculated MW of 42.6kDa according to NP_001814.2). Recommended concentration: 0.1-0.3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CKB |
| Gene ID | 1152 |
| Uniprot ID | KCRB_HUMAN |
| Accession Number | NP_001814.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | KPSDEHKTDLNPDN |
Additional Info
| Function | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work. |
| Protein Name | Creatine Kinase B-TypeBrain Creatine KinaseB-CkCreatine Kinase B ChainCreatine Phosphokinase B-TypeCpk-B |
| Database Links | Reactome: R-HSA-71288Reactome: R-HSA-9696264 |
| Cellular Localisation | CytoplasmCytosolMitochondrionCell MembraneLocalizes To The Mitochondria Of Thermogenic Fat Cells Via The Internal Mts-Like Signal (Imts-L) Region |
| Alternative Antibody Names | Anti-Creatine Kinase B-Type antibodyAnti-Brain Creatine Kinase antibodyAnti-B-Ck antibodyAnti-Creatine Kinase B Chain antibodyAnti-Creatine Phosphokinase B-Type antibodyAnti-Cpk-B antibodyAnti-CKB antibodyAnti-CKBB antibody |
Information sourced from Uniprot.org