Anti-CKAP4 antibody (250-300 aa) (STJ13100963)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 250-300 of mouse CKAP4 conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-CKAP4 (250-300 aa) for use in IHC and WB in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Ckap4 |
| Gene ID | 216197 |
| Uniprot ID | CKAP4_MOUSE |
| Immunogen | A synthetic peptide from aa region 250-300 of mouse CKAP4 conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 250-300 aa |
| Specificity | Specific for CKAP4. |
Additional Info
| Post Translational Modifications | Reversibly palmitoylated. Palmitoylation at Cys-79 by DHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization. Increased phosphorylation during mitosis prevents binding to microtubules. |
| Function | High-affinity epithelial cell surface receptor for APF. Mediates the anchoring of the endoplasmic reticulum to microtubules. |
| Protein Name | Cytoskeleton-Associated Protein 463-Kda Cytoskeleton-Linking Membrane ProteinClimp-63P63 |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-5683826Reactome: -MMU-6798695Reactome: -MMU-8957275Reactome: -MMU-9696264Reactome: -MMU-9696270 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinCell MembraneCytoplasmCytoskeletonPerinuclear RegionTranslocates To The Perinuclear Region Upon Apf-Stimulation |
| Alternative Antibody Names | Anti-Cytoskeleton-Associated Protein 4 antibodyAnti-63-Kda Cytoskeleton-Linking Membrane Protein antibodyAnti-Climp-63 antibodyAnti-P63 antibodyAnti-Ckap4 antibody |
Information sourced from Uniprot.org