• Western blot analysis of extracts of various cell lines, using CISD2 antibody (STJ27203) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CISD2 antibody (61-135) (STJ27203)

SKU:
STJ27203

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CISD2 (61-135) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CISD2
Gene ID: 493856
Uniprot ID: CISD2_HUMAN
Immunogen Region: 61-135
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 61-135 of human CISD2 (NP_001008389.1).
Immunogen Sequence: PKKKQQKDSLINLKIQKENP KVVNEINIEDLCLTKAAYCR CWRSKTFPACDGSHNKHNEL TGDNVGPLILKKKEV
Tissue Specificity Testis, small intestine, kidney, lung, brain, heart, pancreas and platelets.
Function Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca(2+) stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy.
Protein Name Cdgsh Iron-Sulfur Domain-Containing Protein 2
Endoplasmic Reticulum Intermembrane Small Protein
Mitoneet-Related 1 Protein
Miner1
Nutrient-Deprivation Autophagy Factor-1
Naf-1
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Mitochondrion Outer Membrane
It Mainly Localizes To The Endoplasmic Reticulum
However
Experiments In Mouse Showed That It Mainly Localizes To The Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Cdgsh Iron-Sulfur Domain-Containing Protein 2 antibody
Anti-Endoplasmic Reticulum Intermembrane Small Protein antibody
Anti-Mitoneet-Related 1 Protein antibody
Anti-Miner1 antibody
Anti-Nutrient-Deprivation Autophagy Factor-1 antibody
Anti-Naf-1 antibody
Anti-CISD2 antibody
Anti-CDGSH2 antibody
Anti-ERIS antibody
Anti-ZCD2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance