• STJ72322 (0. 01µg/ml) staining of Mouse Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CIRBP antibody (161-172) (STJ72322)

SKU:
STJ72322-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CIRBP (161-172) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.03µg/ml
IHC-3-5µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CIRBP
Gene ID: 1153
Uniprot ID: CIRBP_HUMAN
Immunogen Region: 161-172
Accession Number: NP_001271.1
Specificity: This antibody is expected to recognize reported isoform 1 (NP_001271.1) only.
Immunogen Sequence: RDSYDSYATHNE
Post Translational Modifications Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs. Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
Function Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor. Promotes assembly of stress granules (SGs), when overexpressed.
Protein Name Cold-Inducible Rna-Binding Protein
A18 Hnrnp
Glycine-Rich Rna-Binding Protein Cirp
Cellular Localisation Nucleus
Nucleoplasm
Cytoplasm
Translocates From The Nucleus To The Cytoplasm After Exposure To Uv Radiation
Translocates From The Nucleus To The Cytoplasm Into Stress Granules Upon Various Cytoplasmic Stresses
Such As Osmotic And Heat Shocks
Its Recruitment Into Stress Granules Occurs In The Absence Of Tiar Proteins
Alternative Antibody Names Anti-Cold-Inducible Rna-Binding Protein antibody
Anti-A18 Hnrnp antibody
Anti-Glycine-Rich Rna-Binding Protein Cirp antibody
Anti-CIRBP antibody
Anti-A18HNRNP antibody
Anti-CIRP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance