• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g CIAPIN1 antibody (STJ28258). Western blot was performed from the immunoprecipitate using CIAPIN1 antibody (STJ28258) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using CIAPIN1 Rabbit polyclonal antibody (STJ28258). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CIAPIN1 Rabbit polyclonal antibody (STJ28258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CIAPIN1 antibody (1-312) (STJ28258)

SKU:
STJ28258

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CIAPIN1 (1-312) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CIAPIN1
Gene ID: 57019
Uniprot ID: CPIN1_HUMAN
Immunogen Region: 1-312
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human CIAPIN1 (NP_064709.2).
Immunogen Sequence: MADFGISAGQFVAVVWDKSS PVEALKGLVDKLQALTGNEG RVSVENIKQLLQSAHKESSF DIILSGLVPGSTTLHSAEIL AEIARILRPGGCLFLKEPVE TAVDNNSKVKTASKLCSALT LSGLVEVKELQREPLTPEEV QSVREHLGHESDNLLFVQIT GKKPNFEVGSSRQLKLSITK KSSPSVKPAVDPAAAKLWTL SANDMEDDSMDLIDSDELLD PEDLKKPDPASLRAASCGE
Tissue Specificity Ubiquitously expressed. Highly expressed in heart, liver and pancreas.
Function Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a 4Fe-4S cluster on the scaffold complex NUBP1-NUBP2. Electrons are transferred to CIAPIN1 from NADPH via the FAD- and FMN-containing protein NDOR1. NDOR1-CIAPIN1 are also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit. Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells.
Protein Name Anamorsin
Cytokine-Induced Apoptosis Inhibitor 1
Fe-S Cluster Assembly Protein Dre2 Homolog
Database Links Reactome: R-HSA-2564830
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion Intermembrane Space
Alternative Antibody Names Anti-Anamorsin antibody
Anti-Cytokine-Induced Apoptosis Inhibitor 1 antibody
Anti-Fe-S Cluster Assembly Protein Dre2 Homolog antibody
Anti-CIAPIN1 antibody
Anti-CUA001 antibody
Anti-PRO0915 antibody

Information sourced from Uniprot.org

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