Anti-CHTOP antibody (STJA0008035)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human C1orf77
General Information
| Short Description | Rabbit polyclonal anti-Chromatin target of PRMT1 protein for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHTOP |
| Gene ID | 26097 |
| Uniprot ID | CHTOP_HUMAN |
| Immunogen | Synthesized peptide derived from human C1orf77 |
| Specificity | This antibody detects endogenous levels of C1orf77 at Human |
Additional Info
| Post Translational Modifications | Asymmetrically methylated by PRMT1. Symmetrically methylated by PRMT5. |
| Function | Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis. Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity. |
| Protein Name | Chromatin Target Of Prmt1 ProteinFriend Of Prmt1 ProteinSmall Arginine- And Glycine-Rich ProteinSrag |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | NucleusNucleolusNucleoplasmNucleus SpeckleMostly Associated With Facultative HeterochromatinLocalizes To Regions Surrounding Nuclear Speckles Known As Perispeckles In Which Trex Complex Assembly Seems To Occur |
| Alternative Antibody Names | Anti-Chromatin Target Of Prmt1 Protein antibodyAnti-Friend Of Prmt1 Protein antibodyAnti-Small Arginine- And Glycine-Rich Protein antibodyAnti-Srag antibodyAnti-CHTOP antibodyAnti-C1orf77 antibodyAnti-FOP antibodyAnti-HT031 antibodyAnti-PP7704 antibody |
Information sourced from Uniprot.org