• Western blot analysis of extracts of Mouse brain cells, using Chromogranin B antibody (STJ11103520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using Chromogranin B antibody (STJ11103520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Chromogranin B antibody (550-650) [S0MR] (STJ11103520)

SKU:
STJ11103520

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Chromogranin B (550-650) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHGB
Gene ID: 1114
Uniprot ID: SCG1_HUMAN
Immunogen Region: 550-650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human Chromogranin B (P05060).
Immunogen Sequence: EEENELTLNEKNFFPEYNYD WWEKKPFSEDVNWGYEKRNL ARVPKLDLKRQYDRVAQLDQ LLHYRKKSAEFPDFYDSEEP VSTHQEAENEKDRADQTVLT E
Tissue Specificity Detected in cerebrospinal fluid and urine (at protein level). Expressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver.
Post Translational Modifications Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues. O-glycosylated.
Function Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Protein Name Secretogranin-1
Chromogranin-B
Cgb
Secretogranin I
Sgi Cleaved Into - Pe-11 - Gawk Peptide - Ccb Peptide
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Neuroendocrine And Endocrine Secretory Granules
Alternative Antibody Names Anti-Secretogranin-1 antibody
Anti-Chromogranin-B antibody
Anti-Cgb antibody
Anti-Secretogranin I antibody
Anti-Sgi Cleaved Into - Pe-11 - Gawk Peptide - Ccb Peptide antibody
Anti-CHGB antibody
Anti-SCG1 antibody

Information sourced from Uniprot.org

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