• Western blot analysis of extracts of various cell lines, using Chromogranin A antibody (STJ11103666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Confocal imaging of Human pancreas using Chromogranin A Rabbit monoclonal antibody (STJ11103666, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.
  • Immunohistochemistry of paraffin-embedded rat pancreatic islet using Chromogranin A rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using Chromogranin A Rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using Chromogranin A rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Chromogranin A Rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse pancreatic islet using Chromogranin A rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Chromogranin A Rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat pancreas using Chromogranin A rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Chromogranin A antibody (STJ11103666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of mouse pancreas using Chromogranin A rabbit monoclonal antibody (STJ11103666) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Chromogranin A antibody (1-100) [S6MR] (STJ11103666)

SKU:
STJ11103666

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Chromogranin A (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHGA
Gene ID: 1113
Uniprot ID: CMGA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Chromogranin A (P10645).
Immunogen Sequence: MRSAAVLALLLCAGQVTALP VNSPMNKGDTEVMKCIVEVI SDTLSKPSPMPVSQECFETL RGDERILSILRHQNLLKELQ DLALQGAKERAHQQKKHSGF
Tissue Specificity Detected in cerebrospinal fluid (at protein level). GE-25: Found in the brain.
Post Translational Modifications Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate (CS).CS attachment is pH-dependent, being observed at mildly acidic conditions of pH 5 but not at neutral pH, and promotes self-assembly in vitro. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity. Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides.
Function Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure. Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Protein Name Chromogranin-A
Cga
Pituitary Secretory Protein I
Sp-I Cleaved Into - Vasostatin-1
Vasostatin I - Vasostatin-2
Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin
Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor
Database Links Reactome: R-HSA-6803157
Cellular Localisation Serpinin: Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Pyroglutaminated Serpinin Localizes To Secretory Vesicle
Neuronal Dense Core Vesicle
Secreted
Associated With The Secretory Granule Membrane Through Direct Interaction To Scg3 That In Turn Binds To Cholesterol-Enriched Lipid Rafts In Intragranular Conditions
In Pituitary Gonadotropes
Located In Large Secretory Granules
Alternative Antibody Names Anti-Chromogranin-A antibody
Anti-Cga antibody
Anti-Pituitary Secretory Protein I antibody
Anti-Sp-I Cleaved Into - Vasostatin-1 antibody
Anti-Vasostatin I - Vasostatin-2 antibody
Anti-Vasostatin Ii - Ea-92 - Es-43 - Pancreastatin - Ss-18 - Wa-8 - We-14 - Lf-19 - Catestatin antibody
Anti-Sl21 - Al-11 - Gv-19 - Gr-44 - Er-37 - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor antibody
Anti-CHGA antibody

Information sourced from Uniprot.org

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