Anti-CHRNA3 antibody (90-139 aa) (STJ91447)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human AChRalpha3 at the amino acid range 90-139
General Information
| Short Description | Rabbit polyclonal anti-Neuronal acetylcholine receptor subunit alpha-3 (90-139 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHRNA3 |
| Gene ID | 1136 |
| Uniprot ID | ACHA3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human AChRalpha3 at the amino acid range 90-139 |
| Immunogen Region | 90-139 aa |
| Specificity | AChR Alpha 3 Polyclonal Antibody detects endogenous levels of AChR Alpha 3 protein. |
Additional Info
| Function | Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. CHRNA3 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, with CHRNA5, and CHRNB3 as accesory subunits. CHRNA3:CHRNB4 being predominant in neurons of the autonomic ganglia, it is known as ganglionic nicotinic receptor. CHRNA3:CHRNB4 or CHRNA3:CHRNA5:CHRNB4 play also an important role in the habenulo-interpeduncular tract, modulating the mesolimbic dopamine system and affecting reward circuits and addiction. Hypothalamic CHRNA3:CHRNB4 nAChR activation by nicotine leads to activation of POMC neurons and a decrease in food intake. Also expressed in the urothelium where it modulates reflex bladder activity by increasing intracellular calcium through extracellular influx and basal ATP release. |
| Protein Name | Neuronal Acetylcholine Receptor Subunit Alpha-3 |
| Database Links | Reactome: R-HSA-629587Reactome: R-HSA-629594Reactome: R-HSA-629597 |
| Cellular Localisation | Synaptic Cell MembraneMulti-Pass Membrane ProteinCell MembraneEndoplasmic ReticulumGolgi ApparatusInteraction With Ubxn2a/Ubxd4 Promotes Translocation To The Plasma Membrane |
| Alternative Antibody Names | Anti-Neuronal Acetylcholine Receptor Subunit Alpha-3 antibodyAnti-CHRNA3 antibodyAnti-NACHRA3 antibody |
Information sourced from Uniprot.org