| Host: |
Sheep |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Sheep polyclonal antibody anti-CHMP6 (C-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Chmp6 |
| Gene ID: |
208092 |
| Uniprot ID: |
CHMP6_MOUSE |
| Immunogen Region: |
C-Term |
| Specificity: |
Specific for CHMP6. |
| Immunogen: |
A synthetic peptide from the c-terminal region of mouse CHMP6 conjugated to blue carrier protein was used as the antigen. The antigen shares 95% identity with rat NA s. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes. |
| Protein Name | Charged Multivesicular Body Protein 6Chromatin-Modifying Protein 6 |
| Database Links | Reactome: R-MMU-1632852Reactome: -MMU-5620971Reactome: -MMU-917729Reactome: -MMU-9668328 |
| Cellular Localisation | Endomembrane SystemEndosome MembraneLipid-AnchorLate Endosome MembraneMembraneLocalizes To Endosomal Membranes |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 6 antibodyAnti-Chromatin-Modifying Protein 6 antibodyAnti-Chmp6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
