| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CHMP4B (1-224) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHMP4B |
| Gene ID: |
128866 |
| Uniprot ID: |
CHM4B_HUMAN |
| Immunogen Region: |
1-224 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CHMP4B (NP_789782.1). |
| Immunogen Sequence: |
MSVFGKLFGAGGGKAGKGGP TPQEAIQRLRDTEEMLSKKQ EFLEKKIEQELTAAKKHGTK NKRAALQALKRKKRYEKQLA QIDGTLSTIEFQREALENAN TNTEVLKNMGYAAKAMKAAH DNMDIDKVDELMQDIADQQE LAEEISTAISKPVGFGEEFD EDELMAELEELEQEELDKNL LEISGPETVPLPNVPSIALP SKPAKKKEEEDDDMKELENW AGSM |
| Tissue Specificity | Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. |
| Post Translational Modifications | ISGylated. Isgylation weakens its interaction with VPS4A. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan. (Microbial infection) The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. |
| Protein Name | Charged Multivesicular Body Protein 4bChromatin-Modifying Protein 4bChmp4bSnf7 Homolog Associated With Alix 1Snf7-2Hsnf7-2Vacuolar Protein Sorting-Associated Protein 32-2Vps32-2Hvps32-2 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-1632852Reactome: R-HSA-5620971Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710Reactome: R-HSA-9668328Reactome: R-HSA-9679504Reactome: R-HSA-9694676 |
| Cellular Localisation | CytoplasmCytosolLate Endosome MembranePeripheral Membrane ProteinMidbodyNucleus EnvelopeRecruited To The Nuclear Envelope By Chmp7 During Late AnaphaseLocalizes Transiently To The Midbody Arms Immediately Before Abscission |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 4b antibodyAnti-Chromatin-Modifying Protein 4b antibodyAnti-Chmp4b antibodyAnti-Snf7 Homolog Associated With Alix 1 antibodyAnti-Snf7-2 antibodyAnti-Hsnf7-2 antibodyAnti-Vacuolar Protein Sorting-Associated Protein 32-2 antibodyAnti-Vps32-2 antibodyAnti-Hvps32-2 antibodyAnti-CHMP4B antibodyAnti-C20orf178 antibodyAnti-SHAX1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
