• Immunofluorescence analysis of NIH/3T3 cells using CHMP4B Rabbit polyclonal antibody (STJ29539) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse spleen using CHMP4B Rabbit polyclonal antibody (STJ29539) at 1:400 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-CHMP4B antibody (1-224) (STJ29539)

SKU:
STJ29539

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHMP4B (1-224) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHMP4B
Gene ID: 128866
Uniprot ID: CHM4B_HUMAN
Immunogen Region: 1-224
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CHMP4B (NP_789782.1).
Immunogen Sequence: MSVFGKLFGAGGGKAGKGGP TPQEAIQRLRDTEEMLSKKQ EFLEKKIEQELTAAKKHGTK NKRAALQALKRKKRYEKQLA QIDGTLSTIEFQREALENAN TNTEVLKNMGYAAKAMKAAH DNMDIDKVDELMQDIADQQE LAEEISTAISKPVGFGEEFD EDELMAELEELEQEELDKNL LEISGPETVPLPNVPSIALP SKPAKKKEEEDDDMKELENW AGSM
Tissue Specificity Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.
Post Translational Modifications ISGylated. Isgylation weakens its interaction with VPS4A.
Function Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan. (Microbial infection) The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release.
Protein Name Charged Multivesicular Body Protein 4b
Chromatin-Modifying Protein 4b
Chmp4b
Snf7 Homolog Associated With Alix 1
Snf7-2
Hsnf7-2
Vacuolar Protein Sorting-Associated Protein 32-2
Vps32-2
Hvps32-2
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-1632852
Reactome: R-HSA-5620971
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Reactome: R-HSA-9668328
Reactome: R-HSA-9679504
Reactome: R-HSA-9694676
Cellular Localisation Cytoplasm
Cytosol
Late Endosome Membrane
Peripheral Membrane Protein
Midbody
Nucleus Envelope
Recruited To The Nuclear Envelope By Chmp7 During Late Anaphase
Localizes Transiently To The Midbody Arms Immediately Before Abscission
Alternative Antibody Names Anti-Charged Multivesicular Body Protein 4b antibody
Anti-Chromatin-Modifying Protein 4b antibody
Anti-Chmp4b antibody
Anti-Snf7 Homolog Associated With Alix 1 antibody
Anti-Snf7-2 antibody
Anti-Hsnf7-2 antibody
Anti-Vacuolar Protein Sorting-Associated Protein 32-2 antibody
Anti-Vps32-2 antibody
Anti-Hvps32-2 antibody
Anti-CHMP4B antibody
Anti-C20orf178 antibody
Anti-SHAX1 antibody

Information sourced from Uniprot.org

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