| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CHMP2A (1-222) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHMP2A |
| Gene ID: |
27243 |
| Uniprot ID: |
CHM2A_HUMAN |
| Immunogen Region: |
1-222 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human CHMP2A (NP_055268.1). |
| Immunogen Sequence: |
MDLLFGRRKTPEELLRQNQR ALNRAMRELDRERQKLETQE KKIIADIKKMAKQGQMDAVR IMAKDLVRTRRYVRKFVLMR ANIQAVSLKIQTLKSNNSMA QAMKGVTKAMGTMNRQLKLP QIQKIMMEFERQAEIMDMKE EMMNDAIDDAMGDEEDEEES DAVVSQVLDELGLSLTDELS NLPSTGGSLSVAAGGKKAEA AASALADADADLEERLKNLR RD |
| Post Translational Modifications | ISGylated in a CHMP5-dependent manner. Isgylation weakens and inhibits its interactions with VPS4A and VTA1 respectively. |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. (Microbial infection) The ESCRT machinery functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Involved in HIV-1 p6- and p9-dependent virus release. |
| Protein Name | Charged Multivesicular Body Protein 2aChromatin-Modifying Protein 2aChmp2aPutative Breast Adenocarcinoma Marker Bc-2Vacuolar Protein Sorting-Associated Protein 2-1Vps2-1Hvps2-1 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-1632852Reactome: R-HSA-432720Reactome: R-HSA-5620971Reactome: R-HSA-917729Reactome: R-HSA-9610379Reactome: R-HSA-9615710Reactome: R-HSA-9668328Reactome: R-HSA-9679504Reactome: R-HSA-9694676 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideNucleus EnvelopeLocalizes To The Midbody Of Dividing CellsLocalized In Two Distinct Rings On Either Side Of The Fleming BodyLocalizes To The Reforming Nuclear Envelope On Chromatin Disks During Late Anaphase |
| Alternative Antibody Names | Anti-Charged Multivesicular Body Protein 2a antibodyAnti-Chromatin-Modifying Protein 2a antibodyAnti-Chmp2a antibodyAnti-Putative Breast Adenocarcinoma Marker Bc-2 antibodyAnti-Vacuolar Protein Sorting-Associated Protein 2-1 antibodyAnti-Vps2-1 antibodyAnti-Hvps2-1 antibodyAnti-CHMP2A antibodyAnti-BC2 antibodyAnti-CHMP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
