Western blot analysis of extracts of THP-1 cells, using YKL-40/CHI3L1 antibody (STJ23123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHI3L1 antibody (22-383) (STJ23123)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ23123
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General Information

Short DescriptionRabbit polyclonal YKL-40/CHI3L1 (22-383) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCHI3L1
Gene ID1116
Uniprot IDCH3L1_HUMAN
Immunogen Region22-383
Immunogen SequenceYKLVCYYTSWSQYREGDGSC FPDALDRFLCTHIIYSFANI SNDHIDTWEWNDVTLYGMLN TLKNRNPNLKTLLSVGGWNF GSQRFSKIASNTQSRRTFIK SVPPFLRTHGFDGLDLAWLY PGRRDKQHFTTLIKEMKAEF IKEAQPGKKQLLLSAALSAG KVTIDSSYDIAKISQHLDFI SIMTYDFHGAWRGTTGHHS
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 22-220 of human YKL-40/CHI3L1 (NP_001267.2).

Additional Info

Tissue Specificity Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Post Translational Modifications Glycosylated.
Function Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Protein Name Chitinase-3-Like Protein 1
39 Kda Synovial Protein
Cartilage Glycoprotein 39
Cgp-39
Gp-39
Hcgp-39
Ykl-40
Database Links Reactome: R-HSA-6798695
Cellular Localisation Secreted
Extracellular Space
Cytoplasm
Perinuclear Region
Endoplasmic Reticulum
Alternative Antibody Names Anti-Chitinase-3-Like Protein 1 antibody
Anti-39 Kda Synovial Protein antibody
Anti-Cartilage Glycoprotein 39 antibody
Anti-Cgp-39 antibody
Anti-Gp-39 antibody
Anti-Hcgp-39 antibody
Anti-Ykl-40 antibody
Anti-CHI3L1 antibody

Information sourced from Uniprot.org

Citations

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