Anti-CHFR antibody (Internal) (STJ92263)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the Internal region of human Chfr.
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase CHFR (Internal) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHFR |
| Gene ID | 55743 |
| Uniprot ID | CHFR_HUMAN |
| Immunogen | Synthesized peptide derived from the Internal region of human Chfr. |
| Immunogen Region | Internal |
| Specificity | Chfr Polyclonal Antibody detects endogenous levels of Chfr protein. |
Additional Info
| Post Translational Modifications | Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1. Autoubiquitinated.may regulate its cellular level. Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity. |
| Function | E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. |
| Protein Name | E3 Ubiquitin-Protein Ligase ChfrCheckpoint With Forkhead And Ring Finger Domains ProteinRing Finger Protein 196Ring-Type E3 Ubiquitin Transferase Chfr |
| Database Links | |
| Cellular Localisation | NucleusPml Body |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Chfr antibodyAnti-Checkpoint With Forkhead And Ring Finger Domains Protein antibodyAnti-Ring Finger Protein 196 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Chfr antibodyAnti-CHFR antibodyAnti-RNF196 antibody |
Information sourced from Uniprot.org