Western blot analysis of various lysates using CGRRF1 Rabbit polyclonal antibody (STJ119682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CGRRF1 antibody (40-170) (STJ119682)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ119682
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General Information

Short DescriptionRabbit polyclonal CGRRF1 (40-170) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCGRRF1
Gene ID10668
Uniprot IDCGRF1_HUMAN
Immunogen Region40-170
Immunogen SequenceVPVILRNSEETQFSTRVFKK QMRQVKNPFGLEITNPSSAS ITTGITLTTDCLEDSLLTCY WGCSVQKLYEALQKHVYCFR ISTPQALEDALYSEYLYQEQ YFIKKDSKEEIYCQLPRDTK IEDFGTVPRSR
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 40-170 of human CGRRF1 (NP_006559.1).

Additional Info

Tissue Specificity Ubiquitously expressed with high expression in testis and the cerebellum.
Function Able to inhibit growth in several cell lines.
Protein Name Cell Growth Regulator With Ring Finger Domain Protein 1
Cell Growth Regulatory Gene 19 Protein
Ring Finger Protein 197
Database Links
Cellular Localisation Nucleus
Endoplasmic Reticulum
Alternative Antibody Names Anti-Cell Growth Regulator With Ring Finger Domain Protein 1 antibody
Anti-Cell Growth Regulatory Gene 19 Protein antibody
Anti-Ring Finger Protein 197 antibody
Anti-CGRRF1 antibody
Anti-CGR19 antibody
Anti-RNF197 antibody

Information sourced from Uniprot.org

Citations

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