• Western blot analysis of Hela Cell lysate using Cofilin mouse monoclonal antibody diluted at 1:2,000.
  • Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CFL1 antibody [Mix] (STJ196693)

SKU:
STJ196693

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Cofilin-1 is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Monoclonal
Clone ID: Mix
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFL1
Gene ID: 1072
Uniprot ID: COF1_HUMAN
Specificity: This antibody detects endogenous levels of Cofilin at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from the human Cofilin
Post Translational Modifications Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin.this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.
Function Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
Protein Name Cofilin-1
18 Kda Phosphoprotein
P18
Cofilin - Non-Muscle Isoform
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-399954
Reactome: R-HSA-5627117
Reactome: R-HSA-8950505
Cellular Localisation Nucleus Matrix
Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Lamellipodium Membrane
Lamellipodium
Growth Cone
Axon
Colocalizes With The Actin Cytoskeleton In Membrane Ruffles And Lamellipodia
Detected At The Cleavage Furrow And Contractile Ring During Cytokinesis
Almost Completely In Nucleus In Cells Exposed To Heat Shock Or 10% Dimethyl Sulfoxide
Alternative Antibody Names Anti-Cofilin-1 antibody
Anti-18 Kda Phosphoprotein antibody
Anti-P18 antibody
Anti-Cofilin - Non-Muscle Isoform antibody
Anti-CFL1 antibody
Anti-CFL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance