• Western blot analysis of Rat liver, using CFHR3 rabbit polyclonal antibody (STJ110086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of mouse liver cells using CFHR3 rabbit polyclonal antibody (STJ110086) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of rat liver cells using CFHR3 rabbit polyclonal antibody (STJ110086) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-CFHR3 antibody (80-200) (STJ110086)

SKU:
STJ110086

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CFHR3 (80-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFHR3
Gene ID: 10878
Uniprot ID: FHR3_HUMAN
Immunogen Region: 80-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-200 of human CFHR3 (NP_066303.2).
Immunogen Sequence: AVPCLRKCYFPYLENGYNQN YGRKFVQGNSTEVACHPGYG LPKAQTTVTCTEKGWSPTPR CIRVRTCSKSDIEIENGFIS ESSSIYILNKEIQYKCKPGY ATADGNSSGSITCLQNGWSA Q
Tissue Specificity Expressed by the liver and secreted in plasma.
Function Might be involved in complement regulation.
Protein Name Complement Factor H-Related Protein 3
Fhr-3
Down16
H Factor-Like Protein 3
Database Links Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative Antibody Names Anti-Complement Factor H-Related Protein 3 antibody
Anti-Fhr-3 antibody
Anti-Down16 antibody
Anti-H Factor-Like Protein 3 antibody
Anti-CFHR3 antibody
Anti-CFHL3 antibody
Anti-FHR3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance