• Immunofluorescence analysis of Human liver tissue using Complement factor H Rabbit polyclonal antibody (STJ115641) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of Hep G2 cells using Complement factor H Rabbit polyclonal antibody (STJ115641) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Complement factor H Rabbit polyclonal antibody (STJ115641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CFH antibody (1-240) (STJ115641)

SKU:
STJ115641

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CFH (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFH
Gene ID: 3075
Uniprot ID: CFAH_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human Complement factor H (NP_004485.1).
Immunogen Sequence: MSRSNRQKEYKCGDLVFAKM KGYPHWPARIDEMPEAAVKS TANKYQVFFFGTHETAFLGP KDLFPYEESKEKFGKPNKRK GFSEGLWEIENNPTVKASGY QSSQKKSCVEEPEPEPEAAE GDGDKKGNAEGSSDEEGKLV IDEPAKEKNEKGALKRRAGD LLEDSPKRPKEAENPEGEEK EAATLEVERPLPMEVEKNST PSEPGSGRGPPQEEEEEEDE EEEATKEDAEAPGIRDHES
Tissue Specificity Expressed in the retinal pigment epithelium (at protein level). CFH is one of the most abundant complement components in blood where the liver is the major source of CFH protein in vivo. in addition, CFH is secreted by additional cell types including monocytes, fibroblasts, or endothelial cells.
Post Translational Modifications Sulfated on tyrosine residues.
Function Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
Protein Name Complement Factor H
H Factor 1
Database Links Reactome: R-HSA-977606
Cellular Localisation Secreted
(Microbial Infection) In The Mosquito Midgut
Localizes To P
Falciparum (Nf54 Strain) Macrogamete And Young Zygote Cell Membranes
Alternative Antibody Names Anti-Complement Factor H antibody
Anti-H Factor 1 antibody
Anti-CFH antibody
Anti-HF antibody
Anti-HF1 antibody
Anti-HF2 antibody

Information sourced from Uniprot.org

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