• Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using CETN2 antibody (STJ11104566). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
  • Immunofluorescence analysis of A549 cells using CETN2 antibody (STJ11104566).
  • Western blot analysis of Rat spleen, using CETN2 Rabbit polyclonal antibody (STJ11104566) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CETN2 antibody (1-172) (STJ11104566)

SKU:
STJ11104566

Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Centrin-2 (1-172) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CETN2
Gene ID: 1069
Uniprot ID: CETN2_HUMAN
Immunogen Region: 1-172
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1).
Immunogen Sequence: MASNFKKANMASSSQRKRMS PKPELTEEQKQEIREAFDLF DADGTGTIDVKELKVAMRAL GFEPKKEEIKKMISEIDKEG TGKMNFGDFLTVMTQKMSEK DTKEEILKAFKLFDDDETGK ISFKNLKRVAKELGENLTDE ELQEMIDEADRDGDGEVSEQ EFLRIMKKTSLY
Function Plays a fundamental role in microtubule organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CCP110. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores.
Protein Name Centrin-2
Caltractin Isoform 1
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-3108214
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-5696394
Reactome: R-HSA-5696395
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Nucleus Envelope
Nucleus
Nuclear Pore Complex
Alternative Antibody Names Anti-Centrin-2 antibody
Anti-Caltractin Isoform 1 antibody
Anti-CETN2 antibody
Anti-CALT antibody
Anti-CEN2 antibody

Information sourced from Uniprot.org

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