• Western blot analysis of extracts of various cell lines, using CEP83 antibody (STJ110789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using CEP83 antibody (STJ110789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CEP83 antibody (34-260) (STJ110789)

SKU:
STJ110789

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CEP83 (34-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEP83
Gene ID: 51134
Uniprot ID: CEP83_HUMAN
Immunogen Region: 34-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-260 of human CEP83 (NP_057206.2).
Immunogen Sequence: MLIDERLRCEHHKANYQTLK AEHTRLQNEHVKLQNELKHL FNEKQTQQEKLQLLLEELRG ELVEKTKDLEEMKLQILTPQ KLELLRAQIQQELETPMRER FRNLDEEVEKYRAVYNKLRY EHTFLKSEFEHQKEEYARIL DEGKIKYESEIARLEEDKEE LRNQLLNVDLTKDSKRVEQL AREKVYLCQKLKGLEAEVAE LKAEKENSEAQVENAQRIQV RQLAEMQ
Function Component of the distal appendage region of the centriole involved in the initiation of primary cilium assembly. May collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly.
Protein Name Centrosomal Protein Of 83 Kda
Cep83
Coiled-Coil Domain-Containing Protein 41
Renal Carcinoma Antigen Ny-Ren-58
Database Links Reactome: R-HSA-5620912
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Localizes Specifically To The Distal Appendage Region Of The Centriole
Which Anchors The Mother Centriole To The Plasma Membrane
Localizes To Centrioles At All Stages Of The Cell Cycle
Including Mitosis
Alternative Antibody Names Anti-Centrosomal Protein Of 83 Kda antibody
Anti-Cep83 antibody
Anti-Coiled-Coil Domain-Containing Protein 41 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-58 antibody
Anti-CEP83 antibody
Anti-CCDC41 antibody

Information sourced from Uniprot.org

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