Anti-CEP55 antibody (81-130 aa) (STJ92227)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CEP55 at the amino acid range 81-130
General Information
| Short Description | Rabbit polyclonal anti-Centrosomal protein of 55 kDa (81-130 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CEP55 |
| Gene ID | 55165 |
| Uniprot ID | CEP55_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CEP55 at the amino acid range 81-130 |
| Immunogen Region | 81-130 aa |
| Specificity | CEP55 Polyclonal Antibody detects endogenous levels of CEP55 protein. |
Additional Info
| Post Translational Modifications | There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully. |
| Function | Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation. Plays a role in the development of the brain and kidney. |
| Protein Name | Centrosomal Protein Of 55 KdaCep55Up-Regulated In Colon Cancer 6 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCleavage FurrowMidbodyMidbody RingPresent At The Centrosomes At InterphaseA Small Portion Is Associated Preferentially With The Mother CentrioleWhereas The Majority Localizes To The Pericentriolar MaterialDuring MitosisLoses Affinity For The Centrosome At The Onset Of Prophase And Diffuses Throughout The CellThis Dissociation From The Centrosome Is Phosphorylation-DependentMay Remain Localized At The Centrosome During Mitosis In Certain Cell TypesAppears At The Cleavage Furrow In Late Anaphase And In The Midbody In Cytokinesis |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 55 Kda antibodyAnti-Cep55 antibodyAnti-Up-Regulated In Colon Cancer 6 antibodyAnti-CEP55 antibodyAnti-C10orf3 antibodyAnti-URCC6 antibody |
Information sourced from Uniprot.org