| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CEP55 (1-260) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CEP55 |
| Gene ID: |
55165 |
| Uniprot ID: |
CEP55_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CEP55 (NP_060601.3). |
| Immunogen Sequence: |
MSSRSTKDLIKSKWGSKPSN SKSETTLEKLKGEIAHLKTS VDEITSGKGKLTDKERHRLL EKIRVLEAEKEKNAYQLTEK DKEIQRLRDQLKARYSTTAL LEQLEETTREGERREQVLKA LSEEKDVLKQQLSAATSRIA ELESKTNTLRLSQTVAPNCF NSSINNIHEMEIQLKDALEK NQQWLVYDQQREVYVKGLLA KIFELEKKTETAAHSLPQQT KKPESEGYLQEEKQKCYND |
| Tissue Specificity | Expressed in embryonic brain. Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex. Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain. |
| Post Translational Modifications | There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully. |
| Function | Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation. Plays a role in the development of the brain and kidney. |
| Protein Name | Centrosomal Protein Of 55 KdaCep55Up-Regulated In Colon Cancer 6 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCleavage FurrowMidbodyMidbody RingPresent At The Centrosomes At InterphaseA Small Portion Is Associated Preferentially With The Mother CentrioleWhereas The Majority Localizes To The Pericentriolar MaterialDuring MitosisLoses Affinity For The Centrosome At The Onset Of Prophase And Diffuses Throughout The CellThis Dissociation From The Centrosome Is Phosphorylation-DependentMay Remain Localized At The Centrosome During Mitosis In Certain Cell TypesAppears At The Cleavage Furrow In Late Anaphase And In The Midbody In Cytokinesis |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 55 Kda antibodyAnti-Cep55 antibodyAnti-Up-Regulated In Colon Cancer 6 antibodyAnti-CEP55 antibodyAnti-C10orf3 antibodyAnti-URCC6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
