Anti-CEP131 antibody (311-360 aa) (STJ91793)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human AZI1 at the amino acid range 311-360
General Information
| Short Description | Rabbit polyclonal anti-Centrosomal protein of 131 kDa (311-360 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CEP131 |
| Gene ID | 22994 |
| Uniprot ID | CP131_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human AZI1 at the amino acid range 311-360 |
| Immunogen Region | 311-360 aa |
| Specificity | AZI1 Polyclonal Antibody detects endogenous levels of AZI1 protein. |
Additional Info
| Function | Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Also acts as a negative regulator of BBSome ciliary trafficking. Plays a role in sperm flagellar formation.may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail. Required for optimal cell proliferation and cell cycle progression.may play a role in the regulation of genome stability in non-ciliogenic cells. Involved in centriole duplication. Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. Essential for maintaining proper centriolar satellite integrity. |
| Protein Name | Centrosomal Protein Of 131 Kda5-Azacytidine-Induced Protein 1Pre-Acrosome Localization Protein 1 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentriolar SatelliteCentrioleCilium Basal BodyCytoplasmic VesicleSecretory VesicleAcrosomeColocalized With Pericentriolar Material Protein Pcm1 At Centriolar SatellitesDuring SpermiogenesisBecomes Enriched With Nephrocystin Nphp1 At The Transition ZoneA Structure At The Base Of The Ciliary Axoneme Important For Regulating Traffic Into The Ciliary CompartmentTraffics Towards And Away From The Centrosome/Basal Body And The Transition Zone Of The Ciliary Axoneme In A Microtubule-Dependent MannerLocalized At The Golgi-Derived Acrosome And The Centrosome-Containing Head-Tail Coupling Apparatus (Htca)Ubiquitinated Form Is Sequestered And Colocalized With Bbs4Cep290Pcm1 And Pcnt At Centriolar Satellites In Proliferating CellsColocalized With The Pericentriolar Material Protein Pcm1 At CentrosomeTraffics Towards And Away From Centriolar Satellites And Centrosome In A Microtubule- And Dynein-Dependent Manner In Interphase CellsDisplaced From Centriolar Satellites But Still Remains Associated With The Centrosome In Response To Cellular StressSuch As Ultraviolet Light (Uv) Radiation Or Heat ShockIn A Process That Requires P38 Mapk Signaling |
| Alternative Antibody Names | Anti-Centrosomal Protein Of 131 Kda antibodyAnti-5-Azacytidine-Induced Protein 1 antibodyAnti-Pre-Acrosome Localization Protein 1 antibodyAnti-CEP131 antibodyAnti-AZI1 antibodyAnti-KIAA1118 antibody |
Information sourced from Uniprot.org