Anti-CENTG1/PIKE antibody (Internal) (STJ71901)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CENTG1/PIKE (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 100kDa band observed in Human Brain (Amygdala and Cerebral Cortex) lysates (calculated MW of 90.5kDa according to NP_055585.1). Recommended concentration: 0.3-1ยตg/ml. Primary inc |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | AGAP2 |
| Gene ID | 116986 |
| Uniprot ID | AGAP2_HUMAN |
| Accession Number | NP_055585.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | QASLDSIREAVINSQ |
| Specificity | This antibody is expected to recognize isoform PIKE-S (NP_055585.1). |
Additional Info
| Post Translational Modifications | Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage. |
| Function | GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. |
| Protein Name | Arf-Gap With Gtpase - Ank Repeat And Ph Domain-Containing Protein 2Agap-2Centaurin-Gamma-1Cnt-G1Gtp-Binding And Gtpase-Activating Protein 2Ggap2Phosphatidylinositol 3-Kinase EnhancerPike |
| Database Links | Reactome: R-HSA-373752 |
| Cellular Localisation | Isoform 1: CytoplasmNucleusIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Arf-Gap With Gtpase - Ank Repeat And Ph Domain-Containing Protein 2 antibodyAnti-Agap-2 antibodyAnti-Centaurin-Gamma-1 antibodyAnti-Cnt-G1 antibodyAnti-Gtp-Binding And Gtpase-Activating Protein 2 antibodyAnti-Ggap2 antibodyAnti-Phosphatidylinositol 3-Kinase Enhancer antibodyAnti-Pike antibodyAnti-AGAP2 antibodyAnti-CENTG1 antibodyAnti-KIAA0167 antibody |
Information sourced from Uniprot.org