Anti-CENPE antibody (STJA0007560)

SKU:
STJA0007560

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Centromere-associated protein E is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CENPE
Gene ID: 1062
Uniprot ID: CENPE_HUMAN
Specificity: This antibody detects endogenous levels of CENPE at Human, Mouse
Immunogen: Synthesized peptide derived from human CENPE
Function Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips.after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized. Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss.
Protein Name Centromere-Associated Protein E
Centromere Protein E
Cenp-E
Kinesin-7
Kinesin-Related Protein Cenpe
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-6811434
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Reactome: R-HSA-983189
Cellular Localisation Chromosome
Centromere
Kinetochore
Cytoplasm
Cytoskeleton
Spindle
Associates With Kinetochores During Congression (As Early As Prometaphase)
Relocates To The Spindle Midzone At Anaphase
And Is Quantitatively Discarded At The End Of The Cell Division
Recruited To The Kinetochore In A Sept7
Cenpq And Trappc12-Dependent Manner
Recruited To The Pericentromeric/Centromeric Regions Of The Chromosome In A Ctcf-Dependent Manner
Alternative Antibody Names Anti-Centromere-Associated Protein E antibody
Anti-Centromere Protein E antibody
Anti-Cenp-E antibody
Anti-Kinesin-7 antibody
Anti-Kinesin-Related Protein Cenpe antibody
Anti-CENPE antibody

Information sourced from Uniprot.org

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