Anti-CENPA antibody [R04-9A5] (STJA0033811)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human CENPA
General Information
| Short Description | Rabbit monoclonal anti-CENPA for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-9A5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CENPA |
| Gene ID | 1058 |
| Uniprot ID | CENPA_HUMAN |
| Immunogen | A synthetic peptide of human CENPA |
Additional Info
| Post Translational Modifications | Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway. Trimethylated by NTMT1 at the N-terminal glycine after cleavage of Met-1. Methylation is low before incorporation into nucleosomes and increases with cell cycle progression, with the highest levels in mitotic nucleosomes. Phosphorylated by CDK1 at Ser-68 during early mitosis.this abolishes association with chromatin and centromeres, prevents interaction with HJURP and thereby prevents premature assembly of CENPA into centromeres. Dephosphorylated at Ser-68 by PPP1CA during late mitosis. Phosphorylation of Ser-7 by AURKA and AURKB during prophase is required for localization of AURKA and AURKB at inner centromere and is essential for normal cytokinesis. Initial phosphorylation during prophase is mediated by AURKA and is maintained by AURKB. Poly-ADP-ribosylated by PARP1. |
| Function | Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis. |
| Protein Name | Histone H3-Like Centromeric Protein ACentromere Autoantigen ACentromere Protein ACenp-A |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-606279Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereLocalizes Exclusively To Sites Of Kinetochore Assembly In CentromeresOccupies A Compact Domain At The Inner Kinetochore Plate Stretching Across 2 Thirds Of The Length Of The Constriction But Encompassing Only One Third Of The Constriction Width And HeightPhosphorylation At Ser-68 During Early Mitosis Abolishes Association With Chromatin And Centromeres And Results In Dispersed Nuclear Location |
| Alternative Antibody Names | Anti-Histone H3-Like Centromeric Protein A antibodyAnti-Centromere Autoantigen A antibodyAnti-Centromere Protein A antibodyAnti-Cenp-A antibodyAnti-CENPA antibody |
Information sourced from Uniprot.org