| Short Description : | Rabbit polyclonal CEBPE (1-190) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CEBPE |
| Gene ID: | 1053 |
| Uniprot ID: | CEBPE_HUMAN |
| Immunogen Region: | 1-190 |
| Immunogen Sequence: | MSHGTYYECEPRGGQQPLEF SGGRAGPGELGDMCEHEASI DLSAYIESGEEQLLSDLFAV KPAPEARGLKGPGTPAFPHY LPPDPRPFAYPPHTFGPDRK ALGPGIYSSPGSYDPRAVAV KEEPRGPEGSRAASRGSYNP LQYQVAHCGQTAMHLPPTLA APGQPLRVLKAPLATAAPPC SPLLKAPSPA |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CEBPE (NP_001796.2). |
| Tissue Specificity | Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines. |
| Post Translational Modifications | Phosphorylated. |
| Function | Transcriptional activator. C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation. |
| Protein Name | Ccaat/Enhancer-Binding Protein EpsilonC/Ebp Epsilon |
| Database Links | Reactome: R-HSA-9616222 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ccaat/Enhancer-Binding Protein Epsilon antibodyAnti-C/Ebp Epsilon antibodyAnti-CEBPE antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/96581/379140/STJ117622_1__97810.1713146869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
